Biological preliminaries

Exercises

Here is a picture of the protein Hexokinase.

Find out:

1. What does Hexokinase do?
   (hint: use Swissprot, Genecards, KEGG)
   
   
2. Obtain the sequences of all yeast hexokinase proteins.
   (hint: use Swissprot at expasy)
   
   
3. Obtain the yeast hexokinase A gene.
   (hint: EMBL entry SCHKA , ACcession number X03482 from EBI)
   
   
4. Translate the gene and compare the protein sequence to the swissprot sequences.
   (hint: use ORF Finder at NCBI)
   
   
5. Obtain the genomic DNA of a human hexokinase (AC MMHEXII3 from EBI) and translate it into protein.
   What's the result and why?
   Compare to human protein hexokinase 2.
   (hint: try ORF Finder and GENESCAN (Swissprot -> Proteomics tools...))
   
   
   Relevant webpages:
   
   
   1. Swissprot at expasy
   2. NCBI (ORF Finder)
   3. EBI
   4. Genecards at DKFZ
   5. KEGG

theory

Answers to the exercises.